ISSN 1308-7649 | E-ISSN 2148-3817
Original Article
Tricellulin Expression and its Deletion Effects in the Endolymphatic Sac
1 Department of Otolaryngology, Kagawa University, School of Medicine, Kagawa, Japan  
2 Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectual University of Medicine, Kyoto, Japan  
J Int Adv Otol ; : -
DOI: 10.5152/iao.2018.5473
Key Words: Tricellulin, endolymphatic sac, paracellular transport, tight junction, knockout
Abstract

OBJECTIVES: Tricellulin is a tight junction (TJ)-forming protein that participates in the sealing function of tricellular TJs. Tricellulin-knockout (Tric−/−) mice show progressive hearing loss with degeneration of hair cells in the cochlea without physiological or physical disorders. In the present study, we investigated the tricellulin expression and its deletion effects in the endolymphatic sac (ES) using Tric−/− mice.


MATERIALS and METHODS
: The ES epithelia from wild-type (WT) mice were laser-microdissected, and RT-PCR was performed. The ES sections from Tric−/− and WT mice were immunostained with an anti-tricellulin antibody. Hematoxylin and eosin staining was performed for morphological examination. The inner ear of Tric−/− mice was perfused with biotinylation reagents, and the ES sections were observed for tracer permeability assay after applying streptavidin–Alexa Fluor 488 conjugate.


RESULTS
: The tricellulin expression was confirmed by RT-PCR and by immunohistochemistry in the WT ES. The ES in Tric−/− mice showed normal morphology and revealed no biotin leakage from the lumen.


CONCLUSION
: The ES in Tric−/− mice showed no changes in morphology or disruption in macromolecular barrier function. The effects of solute leakages in the ES of Tric−/− mice may be very limited and compensatable, or that the ES epithelia may have other sealing system covering the lack of tricellulin.


Cite this article as
: Matsubara A, Miyashita T, Inamoto R, Sakaguchi H, Kamitani T, Mori N, et al. Tricellulin Expression and its Deletion Effects in the Endolymphatic Sac. J Int Adv Otol 2018; DOI: 10.5152/iao.2018.5473.

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AVES | Copyright © 2018 The European Academy of Otology and Neurotology | Latest Update: 19.04.2018