ISSN 1308-7649 | E-ISSN 2148-3817
Original Article
Does Short Term Usage of Fresh Pomegranate Juice (FPJ) Protect Cochlear Hair Cells after Cisplatin-Based Chemo-Irradiation?
1 Department of Otolaryngology, Dicle University Faculty of Medicine, Diyarbakır, Turkey  
2 Department of Biophysic, Dicle University Faculty of Medicine, Diyarbakır, Turkey  
3 Department of Pathology, Dicle University Faculty of Medicine, Diyarbakır, Turkey  
4 Department of Chemistry, Dicle University Faculty of Science, Diyarbakır,Turkey  
5 Department of Radiation Oncology, Dicle University Faculty of Medicine, Diyarbakir, Turkey  
6 Department of Biochemistry, Dicle University Faculty of Medicine, Diyarbakır, Turkey  
J Int Adv Otol 2014; 10: 128-133
DOI: 10.5152/iao.2014.72
Key Words: Pomegranate, irradiation, cisplatin-based chemo-irradiation, hair cells of inner ear, ototoxicity, oxidative stress, protein oxidation

OBJECTIVE: The aim of this study was to investigate the protective effect of short-term usage of fresh pomegranate juice (FPJ) on ototoxicity after cisplatin-based chemo-irradiation


MATERIALS and METHODS: The study was carried out on 56 adult Wistar Albino rats, which were divided into 8 groups (n: 7 for each group). The first group was accepted as the sham control group. However, rats in the seven experimental groups were treated with FPJ; cisplatin; irradiation; irradiation plus FPJ; cisplatin plus FPJ; both cisplatin and irradiation; and combined use of cisplatin, irradiation, and FPJ, respectively. Ototoxicity was evaluated by Distortion Product Otoacoustic Emissions (DPOA), histopathology, and paracochlear protein carbonyl content.


RESULTS: The results of the study showed that cisplatin, irradiation, and both cisplatin and irradiation treatments affected hair cells dramatically. However, the results of cisplatin plus FPJ and combined use of cisplatin, irradiation, and FPJ indicated that FPJ plays an important role in protecting hair cells in the inner ear. Additionally, the findings of signal-noise ratio and protein carbonyl values also supported the results stated above.



CONCLUSION: Fresh pomegranate juice treatment can be a supportive agent to reduce hair cell injury in the inner ear of patient treated by cisplatin, radiotherapy, or cisplatin-based chemo-irradiation. However, more performance is necessary for further studies, especially on long-term treatment with FPJ.

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