ISSN 1308-7649 | E-ISSN 2148-3817
Original Article
Angiotensin-Converting Enzyme (ACE) I/D and Alpha-Adducin (ADD1) G460W Gene Polymorphisms in Turkish Patients with Severe Chronic Tinnitus
1 Department of Otorhinolaryngology, Cumhuriyet University School of Medicine, Sivas, Turkey.  
2 Department of Biochemistry, Cumhuriyet University School of Medicine, Sivas, Turkey  
3 Department of Medical Genetics, Cumhuriyet University School of Medicine, Sivas, Turkey  
4 Department of Ear Nose Throat Head and Neck Surgery, Antalya Training and Research Hospital, Antalya, Turkey.  
5 Department of Nutrition and Dietetics, Cumhuriyet University School of Health Sciences, Sivas, Turkey  
J Int Adv Otol 2016; 12: 77-81
DOI: 10.5152/iao.2016.1732
Key Words: Tinnitus, ACE, ADD1, polymorphism

OBJECTIVE: Tinnitus is described as a disturbing sound sensation in the absence of external stimulation. We aimed to investigate whether there is any relationship between severe chronic tinnitus and angiotensin-converting enzyme (ACE) I/D and α-adducin (ADD1) G460W gene polymorphisms.

: The patient group and control group consisted of 89 and 104 individuals, respectively. The evaluation of tinnitus was performed using the Strukturiertes Tinnitus-Interview (STI). The Tinnitus Handicap Inventory (THI) was used to evaluate the tinnitus severity. Polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques were used for genotyping.

: With regard to the ACE I/D polymorphism, there was no significant difference in genotype and allele frequencies between the patient group and control group. However, a statistically significant difference was found in genotype (p<0.01) and allele frequencies (p=0.021) of the ADD1 G460W gene polymorphism. Combined genotype analysis showed that the ACE II /ADD1 GW genotype was statistically significantly higher in the patient group than in the control group (X2: 7.15, p=0.007). The odds ratio value of the GW genotype was 2.5 (95% CI=1.4–4.7) (p<0.01).


CONCLUSION: Our results demonstrate an association between ADD1 G460W gene polymorphism and susceptibility to severe chronic tinnitus. It was found that the GW genotype increased the disease risk by 2.5-fold compared with other genotypes. This indicates that ADD1 G460W polymorphism could be an important factor in the pathophysiology of tinnitus.

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